Fig. 5.
Silencing IQGAP3 and GLI reduced the migration, invasion and stemness potential of lung cancer. (A-B) Representative images and quantified data from wound healing assays conducted in H1299 cells after down-regulating IQGAP3 and GLI1. Scale bar = 200 μm. (C-D) Representative images and quantified data illustrating Transwell migratory assays performed in H1299 and A549 cells after silencing IQGAP3 and GLI1. Scale bar = 50 μm. (E-F) Evaluation of invasion ability in H1299 and A549 cells using Transwell invasive assays after IQGAP3 and GLI1 down-regulation. Scale bar = 50 μm. (G-H) Sphere formation ability of lung cancer cells assessed through sphere formation assays after IQGAP3 and GLI1 silencing. Scale bar = 100 μm. Data represent mean ± SD from experiments conducted in triplicate. **P < 0.01, ***P < 0.001, ns not significant.