Table 6.

Analysis of variance (ANOVA) for response surface model-CCD.

Source	DF	Sum of squares	Mean square	F-Value	P-Value
Model	14	3657.62	261.26	6.06	0.000 (Significant)
Linear	4	2345.43	586.36	13.61	0.000
Temperature (°C)	1	458.15	458.15	10.63	0.005
Tryptone (%)	1	37.75	37.75	0.88	0.363
Inoculum size (%)	1	5.38	5.38	0.12	0.728
Incubation time (hrs)	1	1844.16	1844.16	42.81	0.000
Square	4	821.88	205.47	4.77	0.010
Temperature (°C)* Temperature (°C)	1	338.71	338.71	7.86	0.013
Tryptone (%)*Tryptone (%)	1	281.78	281.78	6.54	0.021
Inoculum size (%)*Inoculum size (%)	1	336.25	336.25	7.81	0.013
Incubation time (hrs)*Incubation time (hrs)	1	106.42	106.42	2.47	0.136
2-Way Interaction	6	490.30	81.72	1.90	0.143
Temperature (°C)*Tryptone (%)	1	2.72	2.72	0.06	0.805
Temperature (°C)*Inoculum size (%)	1	3.05	3.05	0.07	0.794
Temperature (°C)*Incubation time (hrs)	1	480.49	480.49	11.15	0.004
Tryptone (%)*Inoculum size (%)	1	1.38	1.38	0.03	0.860
Tryptone (%)*Incubation time (hrs)	1	0.18	0.18	0.00	0.949
Inoculum size (%)*Incubation time (hrs)	1	2.48	2.48	0.06	0.813
Error	16	689.29	43.08
Lack-of-Fit	10	687.93	68.79	302.46	0.000 (Significant)
Pure Error	6	1.36	0.23
Total	30	4346.91