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. 2025 Jan;31(1):168–173. doi: 10.3201/eid3101.240401

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Emerging Infectious Diseases is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.

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Prion detection using porcine-PMCA in wild pig tissues originating from a CWD-endemic region of Arkansas, USA, in a study of prions in wild pigs (Sus scrofa) from areas with reported chronic wasting disease cases. A) Experimental strategy depicting tissues analyzed in animals from this cohort and PMCA settings. B) Graphs representing percentage of detection per tissue. C) Details on the prion detection data displayed in panel B. PMCA, protein misfolding cyclic amplification; PrP, prion proteins; RPLN, retropharyngeal lymph nodes; SMLN, submandibular lymph nodes; Tg, transgenic.