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. 2025 Jan;31(1):199–202. doi: 10.3201/eid3101.241236

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Emerging Infectious Diseases is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.

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In vitro testing of replication of mammalian influenza A(H5N1) clade 2.3.4.4b isolates against human MxA. MDCK cells overexpressing MxA (MDCK-MxA) or antivirally inactive MxA_T103A (MDCK-MxA_T103A) were infected with an influenza A isolate at a multiplicity of infection of 0.001; viral titers were determined at the indicated time points. A) Pandemic H1N1. B) KAN-1 H5N1. C) White mink H5N1. D) Blue fox H5N1. E) Cat H5N1. F) Bovine H5N1. Data are mean ± SD of n = 3 independent experiments. Dashed line indicates detection limit. hpi, hours postinfection; MxA, human myxovirus resistance protein 1.