Table 2.

Heterologous dosing design.

Group	Prime (Day 0)	Boost (Day 21)
2x saRNA-NLC	saRNA-NLC	saRNA-NLC
2x RBD-3M-052-Alum	RBD-3M-052-Alum	RBD-3M-052-Alum
saRNA-NLC → RBD-3M-052-Alum	saRNA-NLC	RBD-3M-052-Alum
RBD-3M-052-Alum → saRNA-NLC	RBD-3M-052-Alum	saRNA-NLC

Animals were vaccinated i.m. on Days 0 and 21 with the indicated vaccine preparations for each regimen, and serum and tissues were collected on Day 42. saRNA-NLC doses contained 10 μg saRNA per dose. RBD-3M-052-Alum doses contained 7 μg RBD, 2 μg 3M-052, and 100 μg aluminum (Alum) per dose. All vaccinations were prepared in 100 μL and dosed in two 50-μL injections bilaterally in the rear biceps femoris.