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. 2024 Dec 16;15:1486252. doi: 10.3389/fgene.2024.1486252

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Copyright © 2024 Zhang, Meng, Yang, Wang, Zhang, Shi, Guo, Feng and Zeng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Apoptosis of the WT and KO cells under normoxic (A) and DMOG (B) conditions. PI staining (the y-axis) detected DNA content, and Annexin V staining (the x-axis) examined apoptosis, which was represented by the lower right quadrant, corresponding to early apoptotic cells, and the upper right quadrant, corresponding to late apoptotic cells. Percentages of cells in each quadrant were determined automatically by the default settings of the flow cytometry and software and listed inside the graphs. Right panels show the average and standard deviations of the percentages of total apoptotic cells. *: p < 0.05; ***: p < 0.001.