图4.

AURKB通过调控DHX9激活NF-κB信号通路促进骨肉瘤细胞恶性表型

Fig.4 High expression of AURKB enhances malignant phenotype of osteosarcoma cells by activating NF-κB signaling via regulating DHX9. A: EDU experiment for assessing the effects of AURKB silencing and DHX9 overexpression on proliferation of osteosarcoma cells (×100). B: Migration and invasion experiments for assessing the effects of AURKB silencing and DHX9 overexpression on migration and invasion of osteosarcoma cells (×100). C: Western blotting for detecting protein levels of IKBα, p65, phosphorylated IKBα, and phosphorylated p65 in osteosarcoma cells with AURKB silencing and DHX9 overexpression. D: Western blotting for assessing the impact of AURKB silencing and DHX9 overexpression on nuclear translocation of p65. *P<0.05, **P<0.01, ***P<0.001 vs sh-con+Flag-Vector. ^# P<0.05, ^## P<0.01 vs sh-AURKB+Flag-Vector.