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. 2024 Nov 28;104(1):151–163. doi: 10.1111/aogs.15020

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© 2024 The Author(s). Acta Obstetricia et Gynecologica Scandinavica published by John Wiley & Sons Ltd on behalf of Nordic Federation of Societies of Obstetrics and Gynecology (NFOG).

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Comparing results from raw plasma extracellular vesicles (EVs) vs ultracentrifuged pelleted enriched EVs. Green dots to the right 3 columns indicate detection of total EVs using antibodies to CD9, CD63 and CD81 as specific tetraspanins markers of EVs. Red dots at the right three columns marks the placental‐derived EVs detected by anti‐PP13 antibodies. The couple of samples plotted in each shows the signal obtained from the raw plasma preparation vs the pelleted EV sample indicating the reliability and accuracy of the direct plasma samples overlay on the surface of the glass slide of the EV Array.