TABLE 1.
Targeted antigens and antibodies used for the EV Array Analysis. References are provided only for non‐commercial antibodies.
| Targeted antigen | Full name and function | Antibody Clone | Manufacturer |
|---|---|---|---|
| EV Markers | |||
| CD9 | Cluster differentiation 9, Tetraspanin, endosomal membrane markers | SN4/C3‐3A2 | LifeSpan BioSciences, Lynnwood, WA, USA |
| CD63 | Cluster differentiation 63, Tetraspanin, endosomal membrane markers | MEM‐259 | Biolegend, San Diego, CA, USA |
| CD81 | Cluster differentiation 81 Tetraspanin, endosomal membrane markers | 1.3.3.22 | LifeSpan BioSciences, Lynnwood, WA, USA |
| CD82 | Cluster differentiation 82 Tetraspanin, endosomal membrane markers | ||
| Alix | Apoptosis‐linked gene 2‐interacting protein X | 3A9 | Biolegend, San Diego, CA, USA |
| Integrins, immune and coagulation markers | |||
| CD62E/P | Endothelial/Platelet‐selectin, cell adhesion | BBIG‐E(13D5) | R&D system Inc., Minneapolis, MN, USA |
| LFA1 | Lymphocyte function‐associated antigen 1. | HI111 | Abbiotec, Inc., Escondido, CA, USA |
| ICAM1 | Intercellular Adhesion Molecule 1, cell adhesion | R6.5 | eBiosciences, San Diego, CA, USA |
| VEGFR2 | Vascular endothelial growth factor receptor 2, angiogenesis, vessel development and homeostasis | 7D4‐6 | Biolegend, San Diego, CA, USA |
| C1q | Complement component 1q, recognition role in adaptive and innate immunity | 13A16, A201 | Quidel‐Ortho Corporation, San Diego, CA, USA |
| Glypican‐1 | Heparan sulfate proteoglycan | R&D system Inc., Minneapolis, MN, USA | |
| TNF RII | Tumor necrosis factor Receptor II, immunomodulation | 22 210 | R&D system Inc., Minneapolis, MN, USA |
| Placental markers | |||
| PLAP (1) | Placental alkaline phosphatase | 8B6 | Santa Cruz Biotechnologies, Dallas, TX, USA |
| PLAP (2) | Alkaline phosphatase specific to the placenta 5 | NODGE | Oxford group in house mAb |
| PP13 (1) | Specific placental galectin23, 24, 25 | 27‐3‐2 | Hylabs, Rehovot, Israel |
| PP13 (2) | Specific placental galectin23, 24, 25 | 215‐28‐3 | Hylabs, Rehovot, Israel |
| PP13 (3) | Specific placental galectin 24 | 534 | Hylabs, Rehovot, Israel |