Table 1.
Reagent volumes (μL) for the DNAdvance kit based on the manufacturer's protocol (100%) and three adjusted volumes (25%, 33%, and 50%) were used to test differences in DNA yield.
| Percent of manufacturer's suggest volume | ||||
|---|---|---|---|---|
| 25% | 33% | 50% | 100% | |
| Cell Lysis | ||||
| Lysis LBH | 47.0 | 62.0 | 94.0 | 188.0 |
| DTT [1M] | 1.3 | 1.7 | 2.5 | 5.0 |
| Proteinase K [40 mg/μL] | 1.8 | 2.3 | 3.5 | 7.0 |
| DNA isolation | ||||
| Pre-Bind Buffer | 25.0 | 33.0 | 50.0 | 100.0 |
| Bind Buffer | 42.5 | 56.1 | 85.0 | 170.0 |
| Wash | ||||
| Ethyl Alcohol 100% | 3 × 200 | 3 × 200 | 3 × 200 | 3 × 200 |
| Elution | ||||
| Elution EBA Buffer 1X | 30.0 | 30.0 | 30.0 | 30.0 |
Reagents are categorized into cell lysis, DNA isolation, wash, and elution. Note that the volumes for wash and elution were kept constant across all volume treatments