Table 1.
Antibody Reagent List
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| α-SMA | Abcam | Cat: #ab5694 |
| Vimentin | Abcam | Cat#: ab92547 |
| Collagen I |
Proteintech Abcam |
Cat#:67288–1-lg Cat#:ab270993 |
| NR4A1 |
Immunoway Proteintech |
Cat#:YT3213 Cat#:12235–1-AP |
| GAPDH | Proteintech | Cat#:10494–1-AP |
| SPDEF | Proteintech | Cat#:11467–1-AP |
| Chemicals, peptides, and recombinant proteins | ||
| DMEM | Gibco | Cat#:12491,015 |
| Fetal Bovine Serum | Gibco | Cat#:10099 |
| Penicillin and streptomycin | Gibco | Cat#:15070063 |
| Recombinant Human TGF-β1 | Proteintech | Cat#HZ-1011 |
| Adenovirus: Ad-NR4A1 | Hanheng | AP22112703 |
| Adenovirus: Ad-con | Hanheng | AP21072502 |
| Adenovirus: sh-NR4A1 | Hanheng | AD21062310 |
| Adenovirus: sh-con | Hanheng | AP21070906 |
| Adenovirus: Ad-NR4A1 | GenePharma | No.230624AZ |
| Adenovirus: Ad-con | GenePharma | No.E12ZZ |
| Adenovirus: sh-NR4A1 | Genechem | Cat#:127058–1 |
| Adenovirus: sh-con | Genechem | Cat#:LVCON313 |
| Trypsin‑EDTA solution | Gibco | Cat#:25,200,072 |
| Total RNA Kit II | Omega | Cat:#R6934-01 |
| 2*SYBR Green qPCR Master Mix kit | Servicebio | Cat#:G3326-05 |
| Swescript All-in-one First-Strand cDNA Synthesis Super Mix for qPCR | Servicebio | Cat#:G3337-50 |
| HRP-conjugated Affinipure Goat Anti-Rabbit gG (H + L) | Proteintech | Cat#:SA00001-2 |
| HRP-conjugated Affinipure Goat Anti-Mouse gG (H + L) | Proteintech | Cat#:SA00001-1 |
| Mayer, Hematoxylin solution | Solarbio | Cat#:G1080 |
| Histochemistry kit | ZSGB-BIO | Cat#:2221c0725 |
| Normal Goat Serum | Solarbio | Cat#:NO.SL038 |
| DAB | ORIGENE | Cat#:220,030,819 |
| Sodium Citrate buffer | Solarbio | Cat#:C1010 |
| Nonfat dry milk | Biosharp | Cat#:NO.114A0164 |
| Immobilon-E | Millipore | Cat#:R1CB73921 |
| PH8.8 | Solarbio | Cat#:T1010 |
| PH6.8 | Solarbio | Cat#:T1020 |
| ACR | Solarbio | Cat#:CR2209071 |
| TEMED | Solarbio | Cat#:T8090 |
| NcmECL High | NCM Biotech | Cat NO:P10300 |
| CoraLite488-conjugated Goat Anti-Mouse IgG (H + L) | Proteintech | Cat#:No.SA00013-1 |
| CoraLite594 – conjugated Goat Anti-Rabbit IgG (H + L) | Proteintech | Cat#:No.SA00013-4 |