Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2025 Jan 7;31(1):101198. doi: 10.3748/wjg.v31.i1.101198

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/

PMC Copyright notice

The expression of ASH1L in MHCC-97H and knockdown cell lines and proliferation assay, migration and invasion assay of 97H-ASH1L-knockdown. A: Fluorescence imaging was conducted post-lentiviral transfection to visualize the ASH1L protein levels and western blot analysis was performed to compare the protein expression between MHCC-97H Scrambled (Scr) and Knockdown (KD) groups; B: The quantitative analysis of the western blot results provided a numerical comparison of ASH1L protein expression between the MHCC-97H Scr and KD groups; C: Quantitative real-time polymerase chain reaction (RT-qPCR) analysis was utilized to measure the differences in ASH1L gene expression between MHCC-97H Scr and KD groups, providing insights into the transcriptional effects of ASH1L knockdown; D: The colony formation assay indicated that the downregulation of endogenous ASH1L in MHCC-97H cells led to a decrease in the average number of colonies formed, suggesting a reduced clonogenic capacity; E: Cell counting kit 8 assays were employed to assess cell proliferation, and the results demonstrated that the downregulation of endogenous ASH1L significantly decreased the growth rate of MHCC-97H cells; F: Transwell chamber assays were conducted to evaluate and compare the abilities of MHCC-97H Scr and KD cells in terms of migration and invasion, revealing the impact of ASH1L on these cellular behaviors; G: Quantitative analyses of the Transwell assay data statistically compared the migration and invasion capabilities of MHCC-97H Scr and KD cells, further elucidating the role of ASH1L in cell movement and invasion; H: Cell apoptosis was detected by flow cytometry, and the results showed the influence of ASH1L knock-out on apoptosis. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. ^dP < 0.0001. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; Scr: Scrambled; APC: Allophycocyanin conjugate; PE: Phycoerythrin; OD: Optical density.