Table 1.
Primary antibodies used in this study
|
Antibody name
|
Host
|
Brand, category No.
|
Country
|
Applications and dilution
|
||
|
Western blot
|
IHC
|
IF
|
||||
| ACTA2 | Rabbit | Proteintech | China | 1:5000 | 1:800 | 1:100 |
| FN | Rabbit | Proteintech | China | 1:1000 | 1:200 | 1:100 |
| Collagen Ⅰ | Rabbit | Proteintech | China | 1:1000 | 1:500 | 1:100 |
| TGF-β1 | Mouse | Novus | United States | 1:1000 | - | - |
| Smad2 | Rabbit | CST | United States | 1:1000 | - | - |
| Smad3 | Rabbit | CST | United States | 1:1000 | - | - |
| P-Smad2 | Rabbit | Affinity Biotech | United States | 1:1000 | 1:100 | 1:50 |
| P-Smad3 | Rabbit | Affinity Biotech | United States | 1:1000 | 1:100 | 1:50 |
| Vimentin | Rabbit | Proteintech | China | - | - | 1:200 |
| Cytokerin | Rabbit | Abcam | China | - | - | 1:200 |
| GAPDH | Rabbit | CST | United States | 1:1000 | - | - |
ACTA2: Alpha-smooth muscle actin; FN: Fibronectin; p-Smad2/3: Phosphor-Smad2/3; IHC: Immunohistochemistry; IF: Immunofluorescence; CST: Cell signaling technology.