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. 2025 Jan 14;31(2):100898. doi: 10.3748/wjg.v31.i2.100898

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©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/

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Regulator of G protein signaling 4 knockdown inhibited the proliferation, migration, and invasion of gastric cancer cells in vitro. A: The expression levels of regulator of G protein signaling 4 (RGS4) in different gastric cancer (GC) cell lines were detected by real-time quantitative polymerase chain reaction; B: The interference efficiency of the lentiviral human RGS4-targeting short hairpin RNA was validated by real-time quantitative polymerase chain reaction (left) and western blot (right) analysis; C: The proliferation of MGC-803 cells with RGS4 knockdown was determined by cell counting kit-8 assays; D: The effects of RGS4 knockdown on the colony formation of MGC-803 cells; E: The migration ability of MGC-803 cells was measured by wound healing; F: The invasion of MGC-803 cells with RGS4 knockdown was determined by transwell assays. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, NS: No significance. RGS4: Regulator of G protein signaling 4.