Table 2.
Characteristics of the 17 microsatellite loci identified for Ocypode stimpsoni
| Locus (GenBank accession no.) |
Primer sequence (5′-3′) | No. indiv PCR success |
Repeat motif | Size range (bp) | N A | Ho | He | PIC | P HWE | P | Null present |
|---|---|---|---|---|---|---|---|---|---|---|---|
| OS10 | F: 6fam-ACTACTGCTACTACTACCGT | 78 | (TAT)17 | 188–291 | 16 | 0.897 | 0.899 | 0.884 | 0.000* | 0.781 | no |
| (MF461429) | R: CCCCTGATAACCTGTCGACG | ||||||||||
| OS11 | F: hex-GGCGTTATTAGCACTGCTGC | 97 | (ACT)11 | 103–148 | 26 | 0.825 | 0.936 | 0.927 | 0.000* | 1.000 | no |
| (MF461431) | R: TGTTCCTTTCCTTTTTCACTTT | ||||||||||
| OS12 | F: ned-GGCCAGCACAGGTAGAGAAA | 96 | (AGT)21 | 209–281 | 24 | 0.896 | 0.928 | 0.918 | 0.000* | 0.457 | no |
| (MF461432) | R: AGCAGCATCAGGAACAACAA | ||||||||||
| OS13 | F: 6fam-AGCAGCAGTAATAGTAGTAGCAGT | 100 | (CAG)8 | 209–278 | 18 | 0.910 | 0.850 | 0.833 | 0.000* | 0.317 | no |
| (MF461433) | R: GTGTTTCCTTCGCTCAGTGC | ||||||||||
| OS34 | F: pet-TGTCTGTCTGTCTTTTGGTCT | 100 | (TGTC)11 | 120–168 | 14 | 0.960 | 0.825 | 0.802 | 0.005* | 0.000* | no |
| (MF461434) | R: TGACCCGAGGCTTAAAACCC | ||||||||||
| OS36 | F: hex-CCCTAACCCCCTCTCTGTCT- | 100 | (CTAT)8 | 142–186 | 11 | 0.950 | 0.773 | 0.736 | 0.000* | 0.000* | no |
| (MF461435) | R: AACGTGGCAATGCATAACCG | ||||||||||
| OS41 | F: 6fam-GGAACTCTCTCTCAGCACAGG- | 100 | (TATT)7 | 206–234 | 8 | 0.920 | 0.778 | 0.743 | 0.000* | 0.012 | no |
| (MF461436) | R: GAAACACCTGTGCAGCAGTG | ||||||||||
| OS42 | F: 6fam-TTCCTCTCTTCAGCAGCACC | 99 | (TCCT)7 | 162–186 | 7 | 0.788 | 0.783 | 0.746 | 0.263 | 0.054 | no |
| (MF461438) | R: TGGGGATGACAAGAGAGCTG | ||||||||||
| OS43 | F: pet-CTTACGAAGGGGAGAGCGAG | 100 | (AGAC)8 | 225–281 | 14 | 0.850 | 0.873 | 0.855 | 0.000* | 0.594 | no |
| (MF461440) | R: TGTAATCTACCGTGCCCGAG | ||||||||||
| OS46 | F: pet-GGAAGGCAGGTATGGAGAGC | 94 | (AGGA)7 | 270–338 | 17 | 0.734 | 0.890 | 0.876 | 0.000* | 0.998 | no |
| (MF461442) | R: AATCGAAACCAAGCCCTCGT | ||||||||||
| OS47 | F: ned-CGGCGGGTGATTGTAGCTA | 100 | (GTTA)7 | 269–297 | 8 | 0.900 | 0.770 | 0.731 | 0.000* | 0.096 | no |
| (MF461443) | R: GAGCTTTGTCAAGAAGCTGCA | ||||||||||
| OS54 | F: 6fam-TTGCGACTCCAGAAGGTCAC | 99 | (ATAC)10 | 207–235 | 8 | 0.919 | 0.789 | 0.756 | 0.004* | 0.000* | no |
| (MF461444) | R: GCTCCAAGGGCAGAGGTATT | ||||||||||
| OS55 | F: hex-TGGTGGGGATTCGAATAGCG | 76 | (TAAA)7 | 192–208 | 4 | 0.868 | 0.616 | 0.533 | 0.000* | 0.000* | no |
| (MF461445) | R: TGCACCATCCACCCTCATTT | ||||||||||
| OS56 | F: hex-ACCACCCATTCGTCATGTGT | 100 | (CATA)8 | 343–393 | 14 | 0.990 | 0.861 | 0.84 | 0.025 | 0.000* | no |
| (MF461447) | R: GATGATGGACGGGTCGGTTA | ||||||||||
| OS57 | F: pet-GGTCAGGACGGTAATGGCAT | 100 | (GTAT)9 | 280–328 | 14 | 0.890 | 0.865 | 0.846 | 0.019 | 0.866 | no |
| (MF461449) | R: ACGATGAAAACGGCAAAAGTG | ||||||||||
| OS59 | F: ned-CTGACCTGCTGGCTGGTAAA | 96 | (GGCT)8 | 113–149 | 19 | 0.979 | 0.854 | 0.833 | 0.000* | 0.042 | no |
| (MF461450) | R: CACCCCAGCTCAAAGACTCA | ||||||||||
| OS63 | F: ned-CGCAACCTACACAACAGCTG | 88 | (TAAA)7 | 228–296 | 18 | 0.773 | 0.935 | 0.925 | 0.000* | 1.000 | yes |
| (MF461453) | R: GAGTGCTAGGTAGACATGCACA | (0.054) | |||||||||
| Avg | 95.47 | 14.11 | 0.885 | 0.836 | 0.810 | 0.102 | 0.365 | ||||
No indiv PCR success, Number of individuals of PCR success; NA, number of alleles per locus; Ho, observed heterozygosity; He, expected heterozygosity; PIC, polymorphism information content; PHWE, P values of Hardy Weinberg equilibrium test; P, the probability for rejecting Hardy-Weinberg equilibrium when H1 = Heterozygote excess using Genepop (Global Hardy-Weinberg tests); Avg, average. * Indicates significant difference after the application of Holm’s Sequential Bonferroni’s correction (p < 0.05/(17–rank + 1)). The presence of null alleles was investigated using MICROCHECKER v2.2.3 with 1000 randomizations with 95% confidence intervals and 1000 randomizations