Table 3.
Comparison of characteristics of Ocypode stimpsoni microsatellite loci among three populations
| Locus | Yeonggwang (n = 40) | Taean (n = 20) | Seocheon (n = 40) | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No indiv PCR success | N A | Ho | He | PIC |
P
HWE
(P) |
Null present | No indiv PCR success | N A | Ho | He | PIC |
P
HWE
(P) |
Null present | No indiv PCR success | N A | Ho | He | PIC |
P
HWE
(P) |
Null present | |||
| OS10 | 36 | 15 | 0.944 | 0.899 | 0.876 |
0.000* (0.973) |
no | 20 | 12 | 0.900 | 0.909 | 0.876 |
0.398 (0.687) |
no | 22 | 13 | 0.818 | 0.919 | 0.889 |
0.000* (0.420) |
no | ||
| OS11 | 37 | 13 | 0.946 | 0.902 | 0.880 |
0.076 (0.091) |
no | 20 | 10 | 0.950 | 0.881 | 0.844 |
0.172 (0.629) |
Yes (0.150) |
40 | 25 | 0.650 | 0.961 | 0.947 |
0.000* (1.000) |
no | ||
| OS12 | 36 | 17 | 1.000 | 0.907 | 0.885 |
0.014 (0.035) |
no | 20 | 11 | 0.850 | 0.890 | 0.854 |
0.876 (0.159) |
Yes (0.080) |
40 | 20 | 0.825 | 0.903 | 0.885 |
0.000* (0.933) |
no | ||
| OS13 | 40 | 9 | 0.975 | 0.786 | 0.750 |
0.263 (0.923) |
no | 20 | 8 | 0.950 | 0.774 | 0.729 |
0.682 (0.005) |
no | 40 | 15 | 0.825 | 0.892 | 0.87 |
0.001* (0.078) |
no | ||
| OS34 | 40 | 10 | 0.950 | 0.772 | 0.739 |
0.138 (0.023) |
no | 20 | 9 | 1.000 | 0.832 | 0.789 |
0.096 (0.015) |
no | 40 | 9 | 0.950 | 0.822 | 0.788 |
0.624 (0.008) |
no | ||
| OS36 | 40 | 10 | 0.975 | 0.797 | 0.760 |
0.144 (0.009) |
no | 20 | 6 | 1.000 | 0.745 | 0.679 |
0.049 (0.011) |
no | 40 | 7 | 0.900 | 0.770 | 0.723 |
0.003* (0.000*) |
no | ||
| OS41 | 40 | 8 | 0.900 | 0.789 | 0.751 |
0.023 (0.216) |
no | 20 | 8 | 0.800 | 0.785 | 0.743 |
0.137 (0.003*) |
no | 40 | 4 | 1.000 | 0.738 | 0.677 |
0.024 (0.192) |
no | ||
| OS42 | 40 | 6 | 1.000 | 0.818 | 0.780 |
0.035 (0.000*) |
no | 19 | 7 | 0.947 | 0.817 | 0.766 |
0.870 (0.016) |
Yes (0.197) |
40 | 6 | 0.500 | 0.666 | 0.604 |
0.000* (0.956) |
no | ||
| OS43 | 40 | 14 | 0.875 | 0.869 | 0.844 |
0.000* (0.632) |
no | 20 | 9 | 0.750 | 0.872 | 0.833 |
0.000* (0.000*) |
no | 40 | 14 | 0.875 | 0.869 | 0.844 |
0.000* (0.845) |
no | ||
| OS46 | 36 | 11 | 0.944 | 0.838 | 0.811 |
0.682 (0.565) |
no | 20 | 9 | 0.800 | 0.841 | 0.798 |
0.225 (0.024) |
Yes (0.241) |
38 | 13 | 0.500 | 0.869 | 0.842 |
0.000* (1.000) |
no | ||
| OS47 | 40 | 6 | 0.875 | 0.727 | 0.636 |
0.000* (0.886) |
no | 20 | 6 | 0.850 | 0.776 | 0.716 |
0.426 (0.024) |
no | 40 | 8 | 0.950 | 0.783 | 0.740 |
0.045 (0.058) |
no | ||
| OS54 | 40 | 8 | 1.000 | 0.798 | 0.758 |
0.001* (0.000*) |
no | 20 | 8 | 1.000 | 0.810 | 0.767 |
0.139 (0.008) |
no | 39 | 7 | 0.795 | 0.752 | 0.710 |
0.742 (0.571) |
no | ||
| OS55 | 36 | 4 | 0.833 | 0.615 | 0.526 |
0.150 (0.028) |
no | 20 | 4 | 0.900 | 0.638 | 0.545 |
0.318 (0.065) |
no | 20 | 4 | 0.900 | 0.624 | 0.529 |
0.000* (0.000*) |
no | ||
| OS56 | 40 | 11 | 1.000 | 0.863 | 0.834 |
0.444 (0.000*) |
no | 20 | 9 | 1.000 | 0.865 | 0.824 |
0.090 (0.029) |
no | 40 | 11 | 0.975 | 0.864 | 0.835 |
0.832 (0.006) |
no | ||
| OS57 | 40 | 12 | 0.975 | 0.853 | 0.824 |
0.062 (0.929) |
no | 20 | 9 | 1.000 | 0.859 | 0.819 |
0.388 (0.148) |
no | 40 | 12 | 0.750 | 0.874 | 0.848 |
0.064 (0.750) |
no | ||
| OS59 | 36 | 7 | 1.000 | 0.815 | 0.776 |
0.000* (0.000*) |
no | 20 | 6 | 1.000 | 0.735 | 0.671 |
0.266 (0.019) |
no | 40 | 15 | 0.950 | 0.900 | 0.879 |
0.000* (0.842) |
no | ||
| OS63 | 34 | 16 | 0.824 | 0.937 | 0.918 |
0.000* (1.000) |
Yes (0.054) |
19 | 8 | 0.947 | 0.878 | 0.837 |
0.126 (0.601) |
no | 35 | 17 | 0.629 | 0.915 | 0.895 |
0.033 (0.764) |
Yes (0.279) |
||
| Avg | 38.29 | 10.41 | 0.942 | 0.822 | 0.785 |
0.184 (0.371) |
19.88 | 8.17 | 0.920 | 0.818 | 0.770 |
0.328 (0.143) |
37.29 | 11.76 | 0.811 | 0.830 | 0.794 |
0.337 (0.495) |
|||||
No indiv PCR success, Number of individuals of PCR success; NA, number of alleles per locus; Ho, observed heterozygosity; He, expected heterozygosity; PIC, polymorphism information content; PHWE, P values of Hardy Weinberg equilibrium test; P, the probability for rejecting Hardy-Weinberg equilibrium when H1 = Heterozygote excess using Genepop (Global Hardy-Weinberg tests; Avg, average. * Indicates significant difference after the application of Holm’s Sequential Bonferroni’s correction (p < 0.05/(17–rank + 1)). The presence of null alleles was investigated using MICROCHECKER v2.2.3 with 1000 randomizations with 95% confidence intervals and 1000 randomizations