Table 1.

Effect of 32 and 64 mg/L RV in presence or absence of CHX and BZK sub-MICs concentrations, alone or in combination, on biofilm formation in A. baumannii ATCC 19606, 4190 and 3909.

		Strains
	Treatment	ATCC 19606	4190	3909
	TSB	1.02 ± 0.05	0.98 ± 0.02	1 ± 0.02
	32 RV	0.74 ± 0.06	0.89 ± 0.04	0.86 ± 0.02
	64 RV	0.73 ± 0.09	0.84 ± 0.06	0.84 ± 0.1
0 RV	1/8 CHX MIC	0.99 ± 0.01	0.87 ± 0.06	0.9 ± 0.1
	1/4 CHX MIC	0.98 ± 0.03	0.49 ± 0.03**	0.79 ± 0.03
	1/2 CHX MIC	0.87 ± 0.08	0.4 ± 0.1***	0.81 ± 0.03
	1/8 BZK MIC	0.74 ± 0.01	0.69 ± 0.06*	0.8 ± 0.1
	1/4 BZK MIC	0.64 ± 0.1*	0.7 ± 0.04*	0.64 ± 0.03*
	1/2 BZK MIC	0.67 ± 0.04*	0.54 ± 0.03**	0.57 ± 0.03**
	1/8 CHX MIC+1/8 BZK MIC	0.73 ± 0.01	0.57 ± 0.06*	0.46 ± 0.02***
	1/4 CHX MIC+1/4 BZK MIC	0.5 ± 0.03**	0.57 ± 0.03*	0.2 ± 0.01***
	½ CHX MIC+ ½ BZK MIC	0.41 ± 0.03***	0.26 ± 0.02***	0.1 ± 0.03***
32 RV	1/8 CHX MIC	0.75 ± 0.01	0.49 ± 0.06***	0.85 ± 0.06
	1/4 CHX MIC	0.70 ± 0.04	0.44 ± 0.01***	0.77 ± 0.04
	1/2 CHX MIC	0.73 ± 0.04	0.4 ± 0.05***	0.64 ± 0.03
	1/8 BZK MIC	0.71 ± 0.02	0.59 ± 0.06*	0.6 ± 0.03*
	1/4 BZK MIC	0.64 ± 0.03*	0.57 ± 0.03*	0.64 ± 0.02*
	1/2 BZK MIC	0.67 ± 0.02*	0.54 ± 0.01**	0.57 ± 0.03**
	1/8 CHX MIC+1/8 BZK MIC	0.44 ± 0.02***	0.35 ± 0.06***	0.46 ± 0.03***
	1/4 CHX MIC+1/4 BZK MIC	0 ± 0.02***	0 ± 0.02***	0 ± 0.01***
	½ CHX MIC+ ½ BZK MIC	0 ± 0.02***	0 ± 0.01***	0 ± 0.02***
64 RV	1/8 CHX MIC	0.69 ± 0.02*	0.47 ± 0.03***	0.74 ± 0.03
	1/4 CHX MIC	0.56 ± 0.07*	0.4 ± 0.08***	0.65 ± 0.06*
	1/2 CHX MIC	0.46 ± 0.02***	0.39 ± 0.02***	0.52 ± 0.03**
	1/8 BZK MIC	0.59 ± 0.02*	0.56 ± 0.03**	0.6 ± 0.03**
	1/4 BZK MIC	0.58 ± 0.01*	0.54 ± 0.07**	0.57 ± 0.02**
	1/2 BZK MIC	0.57 ± 0.03*	0.44 ± 0.04**	0.55 ± 0.02**
	1/8 CHX MIC+1/8 BZK MIC	0 ± 0.02***	0 ± 0.02***	0 ± 0.02***
	1/4 CHX MIC+1/4 BZK MIC	0 ± 0.01***	0 ± 0.04***	0 ± 0.1***
	½ CHX MIC+ ½ BZK MIC	0 ± 0.02***	0 ± 0.03***	0 ± 0.02***

The results are shown as OD₅₉₅/OD₆₀₀; Synergistic inhibitory effects of RV and/or CHX and BZK on biofilm formation are shown in bold; p-values were calculated using two-way ANOVA (*p < 0.05; **p < 0.01; ***p < 0.001). Assays were performed in triplicate.