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. Author manuscript; available in PMC: 2024 Dec 30.
Published in final edited form as: Methods Mol Biol. 2024;2831:21–37. doi: 10.1007/978-1-0716-3969-6_3

Fig. 2. Analysis of dendritic spines.

Fig. 2.

A. Shown is a micrograph of a representative hippocampal neuron at 17 day in vitro, which was co-labeled with DiI (red) and phalloidin-iFluor 488 (green). Rectangular test areas (20 μm x 10 μm) are superimposed on primary and secondary dendrites with the long axis of the test area oriented parallel to the long axis of the dendrite. B. Shown are high magnification images of a representative test area captured under Fluorescein (top) and Rhodamine (center) filter sets and their merge (bottom). Particular types of dendritic spines are labeled with number, which correspond to the legend given in C. Filopodia and thin spines represent immature spine forms and stain stronger with Phalloidin, while stubby and mushroom spines denote more mature spine forms and more intensely labeled with DiI. C. Shown are morphological features used for classification of dendritic spine types. Scale bar 40 μm in (A) and 5 μm in (B).