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. 2024 Dec 16;15:1520543. doi: 10.3389/fpls.2024.1520543

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Copyright © 2024 Li, Jiang, Li, Liu, Zhang, Wang, Huang, Zhang, Wang, Zhang, Bai and Qin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Metabolite overall profile diagram. (A) TIC overlap Map of quality control samples by mass spectrometry detection;the abscissa represents the retention time (min) of metabolite detection, and the ordinate represents the intensity of the ion current (cps: count per second); (B) Overall sample PC1 control chart. The horizontal coordinate of the graph is the order of sample testing, the vertical coordinate reflects the PC1 value, and the yellow and red lines define a range of plus or minus 2 and 3 standard deviations, respectively. The green dots represent quality control QC samples and the black dots represent test samples; (C) CV distribution graph for each group of samples. The horizontal coordinate represents the CV value, the vertical coordinate represents the proportion of the number of substances smaller than the corresponding CV value to the total number of substances, different colors represent different group samples, and QC is the quality control sample, in which the two reference lines perpendicular to the X-axis correspond to the CV value of 0.3 and 0.5, and the two reference lines parallel to the X-axis correspond to the number of substances accounting for 75% and 85% of the total number of substances; (D) Correlation plot between samples. The vertical and diagonal lines represent the sample names of the different samples, and different colors represent different Pearson correlation coefficients, with redder colors representing stronger positive correlations, greener colors representing weaker correlations, and bluer colors representing stronger negative correlations, and the correlation coefficient magnitudes of the two samples are marked in the boxes; (E) Principal Component Analysis (PCA) plot for the overall sample. pc1 represents the first principal component, pc2 represents the second principal component, pc3 represents the third principal component, and the percentage represents the rate at which that principal component explains the data set; each point in the plot represents a sample, and samples in the same group are represented using the same color, Group is Group; (F) Metabolite categories make up the ring. Each color represents a metabolite category and the area of the color block indicates the proportion of that category.