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. 2024 Dec 16;12:1431420. doi: 10.3389/fbioe.2024.1431420

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Copyright © 2024 Bai, Liu, Gao, Zhang, Niu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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2D Cell co-culture and 3D cytocompatibility. (A) Cell morphology of NSCs and BMSCs co-cultured on the hydrogel surface with different concentrations. (B) LSCM was used to observe the depth of cells entering the scaffold. (C) The activity of co-cultured cells in the hydrogel was detected by Calcein-AM/PI staining. Green fluorescence (AM) indicates viable cells and red fluorescence (PI) indicates dead cells. (D) Analysis of cell migration depth. (E) Assessment of cell viability. (F) Proliferation of cells encapsulated in different concentrations of hydrogels. (n = 3; *p < 0.05, **p < 0.01).