KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-FLAG | Millipore Sigma | cat. # F1804 |
| anti-mouse IgG HRP | Millipore Sigma | cat. #12-349 |
| anti-PER1 | Lee et al., 2001 | GP62 |
| anti-PER2 | Lee et al., 2001 | GP49 |
| anti-guinea pig IgG HRP | Thermo Fisher Scientific | cat. # A18769 |
| anti-Myc agarose conjugate | Santa Cruz Biotechnology | cat. # sc-40 AC |
| α-Myc HRP | Santa Cruz Biotechnology | cat. # sc-40 HRP |
| α-OctA HRP | Santa Cruz Biotechnology | cat. # sc-166355 HRP |
| anti-Myc | Santa Cruz Biotechnology | cat. # sc-40 |
| Rabbit polyclonal phospho-Ser659 | Narasimamurthy et al., 2018 | N/A |
| anti-rabbit IgG HRP | Bio-Rad | cat. # 1706515 |
| anti-mouse IgG HRP | Bio-Rad | cat. # 1706516 |
| Bacterial and virus strains | ||
| Escherichia coli DH5a | NEB | cat. # C2987H |
| Escherichia coli BL21 (DE3) Rosetta2 | Fisher | cat. # 69041 |
| all-in-one CRISPR adenovirus | Jin YH et al., 2019 | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| 2pFASP peptide | Biopeptide Co. | Lot #: 07102013610 |
| 3pFASP peptide | Biopeptide Co. | Lot #: 07102013611 |
| 4pFASP peptide | Biopeptide Co. | Lot #: 09082013881 |
| no_p peptide | Biopeptide Co. | Lot #: 11092115443 |
| pS589 peptide | Biopeptide Co. | Lot #: 11092115440 |
| pS596 peptide | Biopeptide Co. | Lot #: 11092115441 |
| pS589/pS596 peptide | Biopeptide Co. | Lot #: 11092115442 |
| HGS-CK1δ (1–294) | This study | N/A |
| HGST-CK1δ (1–317) | This study | N/A |
| HGST-CK1δ (1–317) K224D | This study | N/A |
| HNXL-PER1 FASP | This study | N/A |
| HNXL-PER1 FASP Indel P1E17-1 | This study | N/A |
| HNXL-PER1 FASP Indel P1E17-38 | This study | N/A |
| HNXL-PER2 FASP | This study | N/A |
| HNXL-PER2 FASP S662A | This study | N/A |
| HNXL-PER2 FASP S665A | This study | N/A |
| HNXL-PER2 FASP S668A | This study | N/A |
| HNXL-PER2 FASP S671A | This study | N/A |
| HNXL-PER2 FASP S674A | This study | N/A |
| HNXL-PER2 FASP S662D | This study | N/A |
| HNXL-PER2 PAS-Degron | This study | N/A |
| HNXL-PER2 CK1BD | This study | N/A |
| HNS-PER2 Degron-CK1BD-B TEV507 | This study | N/A |
| HNS-PER2 Degron-CK1BD-B TEV507 S662G | This study | N/A |
| His-TEV | Blommel et al., 2007 | N/A |
| Critical commercial assays | ||
| ADP-Glo | Promega | cat. # V9102 |
| Deposited data | ||
| Human FASP chemical shift assignments | This study | BMRB: 51827 |
| CK1δ:2pFASP structure | This study | PDB: 8D7M |
| CK1δ:3pFASP structure | This study | PDB: 8D7N |
| CK1δ:4pFASP structure | This study | PDB: 8D7O |
| CK1δ:pS589 structure | This study | PDB: 8D7P |
| Experimental models: Cell lines | ||
| Human: U2OS cells | ATCC | cat. # HTB-96 |
| Human: HEK293T cells | ATCC | cat. # CRL-3216 |
| D. melanogaster: S2 cells | Thermo Fisher Scientific | cat. # R69007 |
| Oligonucleotides | ||
| See Supplementary Table 4 | Millipore Sigma | N/A |
| Recombinant DNA | ||
| pET-22b(+) HGS-CK1δ (1–294) | This study | N/A |
| pET-22b(+) HGST-CK1δ (1–317) | This study | N/A |
| pET-22b(+) HGST-CK1δ K224D | This study | N/A |
| pET-22b(+) HNXL-PER2 FASP | This study | N/A |
| pET-22b(+) HNXL-PER2 FASP S662A | This study | N/A |
| pET-22b(+) HNXL-PER2 FASP S665A | This study | N/A |
| pET-22b(+) HNXL-PER2 FASP S668A | This study | N/A |
| pET-22b(+) HNXL-PER2 FASP S671A | This study | N/A |
| pET-22b(+) HNXL-PER2 FASP S674A | This study | N/A |
| pET-22b(+) HNXL-PER2 FASP S662D | This study | N/A |
| pET-22b(+) HNXL-PER2 PAS-Degron | This study | N/A |
| pET-22b(+) HNXL-PER2 CK1BD | This study | N/A |
| pET-22b(+) HNXL-PER1 FASP | This study | N/A |
| pET-22b(+) HNXL-PER1 FASP Indel P1E17-1 | This study | N/A |
| pET-22b(+) HNXL-PER1 FASP Indel P1E17-38 | This study | N/A |
| pAc-3XFLAG-His-dper/Tev100-6Xc-myc | Chiu et al., 2008 | N/A |
| pAc-dper WT (aa1-1224)-V5 | Chiu et al., 2011 | N/A |
| pAc-dper S596A (aa1-1224)-V5 | Chiu et al., 2011 | N/A |
| pAc-dper WT (aa560-1034)-V5 | Ko et al., 2010 | N/A |
| pCS MT-PER2 | This study | N/A |
| pCS MT-PER2 S662A | This study | N/A |
| pCS MT-PER2 S665A | This study | N/A |
| pCS MT-PER2 S668A | This study | N/A |
| pCS MT-PER2 S671A | This study | N/A |
| pCS MT-PER2 S674A | This study | N/A |
| pCS MT-PER2 S480A | This study | N/A |
| pcDNA4B-FLAG-CK1δ FLAG | This study | N/A |
| pcDNA4B-FLAG-CK1δ R178C (tau) FLAG | This study | N/A |
| pcDNA4B-β-TrCP | This study | N/A |
| all-in-one pAdTrack-Cas9-DEST | Jin YH et al., 2019 | N/A |
| Software and algorithms | ||
| PRISM | GraphPad | |
| Mathematica 11.0 | Wolfram Research | |
| ImageJ | NIH | 2.0.0-rc-67/1.52d |
| AMBER16 | Case, 2016 | |
| Maestro | Schrodinger | 2020-3 |
| PrepWizard | See method details | |
| PHENIX | Adams et al., 2011 | 1.13-2998 |
| Coot | CCP4 | 0.8.9.2 EL |
| PyMOL | Schrodinger LLC. | 2.1.1 |
| NMRPipe | Delaglio et al., 1995 | |
| CCPNmr Analysis | Vranken et al., 2005 | 2.5.2 |
| Excel | Microsoft | 16.61.1 |
| Other | ||