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. 2024 Dec 30;14:31680. doi: 10.1038/s41598-024-81131-5

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2024

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Fig. 5 — Estimating the limit of detection (LOD) of the multiplex HRM assay. Derivative melt plots of a ten-fold dilution series of template DNA (20, 2, 0.2, 0.02 ng) using DNA derived from (a) 15-ADON (strain PH-1), (b) 3-ADON (isolate 00-500), (c) NIV (isolate 02–15) or (d) NX-2 (isolate 06-156) reference isolates. A fifth concentration of 0.002 ng was used for all isolates but failed to amplify suggesting a LOD between 0.02 and 0.002 ng.