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Comparative analysis of bacterially expressed and refolded and purified recombinant EcAMA1 with Pichia-expressed recombinant PpAMA1. Molecular mass markers are shown in kDa. Samples were run on 4 to 20% SDS-PAGE under nonreduced or reduced and alkylated (R/A) conditions. A, Coomassie blue stain analysis. B, Western blot developed using anti-AMA1 4G2 monoclonal antibody.
