FIG. 1.

Fiber and secondary inclusion formation in cells infected by Chlamydia trachomatis. The four panels show the different forms of inclusions found within McCoy cells infected with C. trachomatis G/UW-57. Cells were infected at an MOI of 0.3 and fixed with methanol 28 hpi. All cells were labeled with anti-IncA (green) and anti-MOMP (red), and DNA is labeled with DAPI (blue). (A) Two infected cells, one containing an inclusion lacking a fiber and one containing an inclusion with a single IncA-laden fiber. (B) A cell with an IncA-laden vacuole at the end of a fiber. (C) A cell with a maturing primary inclusion, a secondary inclusion containing a limited number of developmental forms, and a connecting fiber. (D) An infected cell with two large inclusions, both filled with developmental forms and connected by an IncA-laden fiber. Scale bar, 10 μm.