FIG. 5.

IncA-laden secondary inclusions exclude the green fluorescence protein. Chlamydia trachomatis G/UW-57 was used to infect McCoy cells (MOI = 0.5) previously transfected with pCDNA-GFP. The cells were fixed for conventional immunofluorescence microscopy 30 hpi. (A) An infected cell that is producing GFP (green) and is labeled with anti-IncA (red) and DAPI (DNA; blue). A primary and two secondary inclusions can be seen in this cell. (B) GFP labeling of the same cell. Note that the primary inclusion, the filled secondary inclusion (thin arrows), and the secondary inclusion lacking chlamydiae (fat arrow) all exclude GFP. Scale bar in panel B, 10 μm.