FIG. 3.

H2-M3-restricted CD8⁺ T-cell expansion following L. monocytogenes fMIVTLF^neg infection. (A) Six days following infection with L. monocytogenes (L.m.) 10403s or L. monocytogenes fMIVTLF^neg, the total number of fMIGWII- or fMIVTLF:H2-M3-tetramer-positive CD8⁺ cells was determined. (B and C) The total number of CD8⁺ T cells producing gamma interferon (IFN-γ) (B) or tumor necrosis factor alpha (TNF-α) (C) 6 days following infection with L. monocytogenes 10403s or L. monocytogenes fMIVTLF^neg were determined by ex vivo intracellular cytokine staining. non-inf., noninfected. (D) fMIVTLF-specific in vivo cytolysis is induced by immunization with L. monocytogenes fMIVTLF^neg infection. The numbers above the graphs refer to individual mice used in one experiment. The experiment was repeated twice.