Skip to main content
. 2005 Jul;73(7):3912–3922. doi: 10.1128/IAI.73.7.3912-3922.2005

FIG. 5.

FIG. 5.

PfMSP-8 localizes to the parasitophorous vacuole of ring-stage parasites and is absent from the surface of merozoites. (A) Double-labeling IFA of fixed early-ring-stage, late-ring-stage, and schizont-stage D10-MSP-8 3′ and D10-ΔMSP-8 parasites, using a mixture of rabbit antisera specific for the EGF-like domains of PfMSP-8 (αPfMSP-8) and monoclonal antibody 4H9/19 specific for PfMSP-119 (αPfMSP-1). DAPI was used for nuclear staining (blue). PfMSP-8 (green) and PfMSP-1 (red) colocalized in the parasitophorous vacuole of early-ring-stage P. falciparum parasites but not in the parasitophorous vacuole of late-stage P. falciparum parasites. (B) Double-labeling IFA of fixed merozoite-stage D10-MSP-8 3′ and D10-ΔMSP-8 parasites, using a mixture of rabbit antisera specific for the EGF-like domains of PfMSP-8 and monoclonal antibody 4H9/19 specific for PfMSP-119 as described above. schiz, schizont.