FIG. 2.

Immunofluorescent staining of C. trachomatis Tarp-enhanced GFP (EGFP) constructs for tyrosine phosphorylation. The constructs described in the legend to Fig. 1 were expressed in HeLa 229 cells and stained for tyrosine phosphorylation using MAb 4G10 with an Alexa Fluor 594-conjugated anti-mouse IgG secondary antibody or stained for actin recruitment using Texas Red phalloidin. Full-length Tarp and the C-terminal domain form aggregates in the host cell. The N-terminal domain and repeat region show diffuse localization throughout the cytoplasm and nucleus. CT229, an inclusion membrane protein (1, 34) expressed as a negative control, also forms aggregates within the cytoplasm. Bar, 10 μm.