FIG. 4.

Actin is recruited to the site of entry by C. trachomatis, C. muridarum, C. caviae, and C. pneumoniae EBs. HeLa 229 cells expressing GFP-actin were infected with CMTMR-labeled (red) C. trachomatis serovars L2 and D, C. muridarum (MoPn), C. caviae (GPIC), and C. pneumoniae (Cpn) EBs and examined by spinning disk confocal microscopy for recruitment of actin. All chlamydial species and serovars examined demonstrated robust but transient recruitment of actin to the site of entry. The figure is a composite of single frames taken from a time-lapse series for each strain or species. Bar, 5 μm.