FIG. 7.

C. trachomatis Tarp secreted from Yersinia pseudotuberculosis is translocated via type III secretion into HeLa cells and tyrosine phosphorylated. Immunoblots of HeLa cells infected with Yersinia pseudotuberculosis MEKA (translocation competent) and MEKBA (translocation incompetent) vector controls or expressing C. trachomatis L2 full-length Tarp or Tarp with the repeat domain deleted are shown. Whole-cell lysates were probed for tyrosine phosphorylation with MAb 4G10 (A) or for Tarp expression with polyclonal rabbit anti-Tarp antibody (B). Asterisks indicate the positions of full-length Tarp; the diamond shows the position of the truncated product.