TABLE 1.

Bacterial strains used in this study

Strain	Description or origin	Source or reference
Escherichia coli DH5α	F− φ80lacZΔM15 Δ(lacZYA-argF) U169 endA1 recA1 hsdR17 (rk−, mk+) supE44 thi-1 gyrA96 relA1 phoA	Invitrogen
P. aeruginosa
PAK	Wild-type laboratory strain	D. Bradley
PAO1	Wild-type laboratory strain	M. Vasil
JJ692	UTI isolate	S. Lory
PAKΔC	fliC deletion strain of P. aeruginosa PAK	8
PAOC−	Gentamicin insertion mutant in the fliC gene of P. aeruginosa PAO1	11
PAKmotABCD	Both sets of mot genes, motAB and motCD deleted from P. aeruginosa PAK	This study
PAKrfbC	Gentamicin insertion mutant in the rfbC gene of P. aeruginosa PAK	3
PAOrfbC	Gentamicin insertion mutant in the rfbC gene of P. aeruginosa PAO1	This study
PAKΔC + PAKfliC	PAKΔC complemented on the chromosome with the PAK fliC gene at the attB site	This study
PAKΔC + PAO1fliC	PAKΔC complemented on the chromosome with the PAO1 fliC gene at the attB site	This study
PAKΔC + JJ692fliC	PAKΔC complemented on the chromosome with the fliC gene from the P. aeruginosa clinical strain JJ692 at the attB site	This study
PAOC− + PAO1fliC	PAOC− complemented on the chromosome with the fliC gene from the P. aeruginosa PAO1 fliC gene at the attB site	This study