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The first 20 amino acids of SepZ can direct translocation of a β-lactamase fusion into HeLa cells. Images presented are of HeLa cell monolayers loaded with fluorescent β-lactamase substrate CCF2/AM. HeLa cells had been previously left uninfected, infected with MK41(pKK502), or infected with MK41(pGST-BlaM) (reading from left to right, respectively).
