TABLE 2.

Primers used in this study (sequences 5′-3′)

Function and primer	Sequence
SepZ-CyaA fusion construction
SepZ-C-F	CCCAAGCTTCTAATAAATGTCTAAATTAGACAAAAGG
SepZ-C-R	CGCGGATCCGGCATATTTCATCGCTAATCCG
MK41 construction
SepZ-M-F1	CCGGAATTCCTGCGGGCATATCACCATCAATACTCA
SepZ-M-R1	ACAGTAGTTACCCGGGGCTGCTTCCATTGATCTTTCTCCT
SepZ-M-F2	TGGAAGCAGCCCCGGGTAACTACTGTTGCCGGCGGATTA-GCGAT
SepZ-M-R2	CGCGGATCCTGAATGACCGATGGTGCTAAGTTAACC
β-Lactamase fusion construction
BlaM-F	CCTTCCTGTTGGATCCCACCCAGAAAC
BlaM-R	ATGAGTAAACGAATTCTGACAGTTAC
SepZ-B-R	CGCGGATCCATTGATTGTGGCTGCCAGTGGTAATACTGCACCA-GAAGGGCTTAAATTTGCTGCTTCCATGAATACTGTTTCCTGTG
GEX-B-F	ATCTGGTTCCGCGTGGATCC
GST-SepZ fusion construction
SepZ-G-F	CGCGGATCCGAAGCAGCAAATTTAAGCCC
SepZ-G-R	CCGGAATTCTTAGGCATATTTCATCGCTAATC