FIG. 4.

Semiquantitative RT-PCR confirms the mRNA expression profiles of indoleamine-1,2-dioxygenase, intelectin, CXCL9, and CXCL11 as revealed by microarray. RT-PCR analyses of the expression levels of (A) indoleamine-1,2-dioxygenase (Indo), (B) CXCL11, (C) CXCL9, and (D) intelectin (Itln) at day 19 postinfection in gut tissue from AKR and BALB/c mice are shown. cDNA for HPRT was titrated out, and nonsaturated dilutions giving bands of equivalent brightness, shown as a single band per sample in the figure, were selected as the starting dilution for the serial dilutions. cDNA was serially diluted (1:2) and amplified by PCR as described in Materials and Methods. A, AKR; B, BALB/c. N, naïve; 19, day 19 postinfection.