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. 2005 Jul;73(7):4205–4213. doi: 10.1128/IAI.73.7.4205-4213.2005

FIG. 1.

FIG. 1.

CAMP test with B. henselae and E. coli XL1-Blue MR recombinant strains. Strains were grown perpendicular to the S. aureus streak (b). The diffusion zone of the sphingomyelinase produced by S. aureus is indicated with two arrows (a). The B. henselae strain shows the CAMP effect with increased hemolysis next to the S. aureus streak (lane 1). The group B streptococcus control strain shows a large distinct zone with complete hemolysis next to the indicator strain (lane 2). The Listeria monocytogenes control strain shows a mild CAMP reaction (lane 3). The E. coli XL1-Blue MR strain containing the SuperCos1 cosmid vector shows no CAMP reaction (lane 4). The E. coli XL1-Blue strain containing the SuperCos1 cosmid pCML75 with the 29.7-kb insert of B. henselae chromosomal DNA shows a mild CAMP effect (lane 5). The E. coli XL1-Blue strain containing plasmid pCML76 with the 7.2-kb subclone with the entire cfa gene and promoter shows a distinct zone of complete hemolysis next to the S. aureus streak (lane 6).