FIG. 2.

Restriction map of the region near the cfa gene and positions of clones. The arrows represent the cfa gene with the arrowhead showing the direction of transcription. The diagonal hatch marks at the arrowhead represent the area of homology of the translated protein with the β-barrel region of proteins in the family of autotransporters. The grey area and vertical bars in the stem of the arrow represent homology of the translated protein with RTX (repeat in toxin) hemolysins. The vertical bars represent the four tandem nearly identical repeats in the amino acid sequence. Plasmid pCML77 contains the StuI-BamHI insert from pCML76 (solid dark line) with an internal 5.6-kb EcoRV-EcoRV deletion (open line) of the coding sequence of cfa.