TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Bacterial strains | ||
| B. henselae | Isolated from blood of a 31-year-old male with AIDS, Oklahoma City, OK, ATCC 49793 | ATCC |
| Staphylococcus aureus | Sphingomyelinase-producing strain used for CAMP test for Listeria monocytogenes, ATCC 25923 | ATCC |
| Group B streptococcus | Control strain used for CAMP test | Lab stock |
| Listeria monocytogenes | Control strain used for CAMP test | Lab stock |
| E. coli XL1-Blue MR | ΔmcrA183 ΔmcrCB-hsdSMR-mrr endA1 supE44 thi-1 recA1 gyrA96 relA1 lac | 5 |
| Plasmids | ||
| SuperCos1 | Cosmid cloning vector; Kmr, Apr | Stratagene |
| pCML75 | B. henselae cfa cosmid clone from B. henselae ATCC 49793 genomic library; 29.7-kb chromosomal fragment in SuperCos1; Kmr, Apr | This study |
| pCML76 | B. henselae cfa subclone of pCML75 containing 7.2-kb StuI-BamHI cfa clone in a plasmid derivative of SuperCos1 with 10× higher copy number than original cosmid; SuperCos1 derivative has a 3.4-kb fragment deleted from StuI site within SuperCos1 to the StuI site in cfa clone; the simian virus 40 promoter sequence and cos sequences were deleted; Kmr, Apr | This study |
| pCML77 | pCML76 with an internal 5.6-kb EcoRV-EcoRV deletion of the coding sequence of cfa; Kmr, Apr |
a
Kmr, kanamycin resistance; Apr, ampicillin resistance.