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. 2005 Jul;73(7):4098–4105. doi: 10.1128/IAI.73.7.4098-4105.2005

FIG. 4.

FIG. 4.

Identification of Nfa1 protein from a lysate by Western blotting (after 15% SDS-PAGE) with an anti-Nfa1 polyclonal antibody. The lysate of N. gruberi transfected with the pEGFP-C2/nfa1UTR vector was prepared 3 months after G418 treatment. (A and B) Coomassie blue staining after SDS-PAGE and immunoblotting, respectively. Lanes PM, prestained marker; lanes 1, N. gruberi transfected with the pEGFP-C2/nfa1UTR vector; lanes 2, N. fowleri; lanes 3, N. gruberi; lanes 4, N. gruberi transiently transfected with the pEGFP-C2/nfa1 vector.