TABLE 1.
Primer | Sequence | Cloned vector | Enzyme site |
---|---|---|---|
nfa1 F1 | 5′-GAGCTCAAGCTTTATACATATGCGG-3′ | pEGFP-C2/nfa1 | HindIII |
nfa1 R1 | 5′-TAGTTATTGCTCAGCGGTGG-3′ | pEGFP-C2/nfa1 | EcoRI |
nfa1UTR F1 | 5′-ACTCAGATCTCCGAATAGTAGCACCAC-3′ | pEGFP-C2/nfa1UTR | BglII |
nfa1UTR R1 | 5′-CCCGGAATTCCGATCATACACAATCATC-3′ | pEGFP-C2/nfa1UTR | EcoRI |