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. 2005 Jul;73(7):4098–4105. doi: 10.1128/IAI.73.7.4098-4105.2005

TABLE 1.

Primers used to clone the vectors for transfection

Primer Sequence Cloned vector Enzyme site
nfa1 F1 5′-GAGCTCAAGCTTTATACATATGCGG-3′ pEGFP-C2/nfa1 HindIII
nfa1 R1 5′-TAGTTATTGCTCAGCGGTGG-3′ pEGFP-C2/nfa1 EcoRI
nfa1UTR F1 5′-ACTCAGATCTCCGAATAGTAGCACCAC-3′ pEGFP-C2/nfa1UTR BglII
nfa1UTR R1 5′-CCCGGAATTCCGATCATACACAATCATC-3′ pEGFP-C2/nfa1UTR EcoRI