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. 2005 Jul;73(7):4098–4105. doi: 10.1128/IAI.73.7.4098-4105.2005

TABLE 2.

Primers used for the PCR of gDNA or cDNA

Primer Sequence Position bp DNA amplified DNA
GFP F1 5′-ACAACATCGAGGACGGCAGCGTGCAGCTCG-3′ 1121-1150 in pEGFP-C2 Fragment between GFP and nfa1 cDNA
GFP F2 5′-CATGGTCCTGCTGGAGTTCGTG-3′ 337-360 in nfa1 Fragment between GFP and nfa1 cDNA
nfa1 F2 5′-ATGGCACTACTATTCCATCACCA-3′ 1-24 in nfa1 nfa1 gDNA
nfa1 R2 5′-TTAAAGCACTCCCTTGTACTTCAT-3′ 1166-1287 in pEGFP-C2 nfa1UTR gDNA
nfa1 R3 5′-AACTCTTCACGAGCAAATGCCAAACGCTTTAAAGCAC-3′ 361-397 in nfa1UTR nfa1UTR gDNA
VS1 5′-GTTTGGACAAACCACAACTAGAATGCAGTG-3′ 1599-1628 in pEGFP-C2 nfa1 gDNA