TABLE 2.
Primer | Sequence | Position bp | DNA amplified | DNA |
---|---|---|---|---|
GFP F1 | 5′-ACAACATCGAGGACGGCAGCGTGCAGCTCG-3′ | 1121-1150 in pEGFP-C2 | Fragment between GFP and nfa1 | cDNA |
GFP F2 | 5′-CATGGTCCTGCTGGAGTTCGTG-3′ | 337-360 in nfa1 | Fragment between GFP and nfa1 | cDNA |
nfa1 F2 | 5′-ATGGCACTACTATTCCATCACCA-3′ | 1-24 in nfa1 | nfa1 | gDNA |
nfa1 R2 | 5′-TTAAAGCACTCCCTTGTACTTCAT-3′ | 1166-1287 in pEGFP-C2 | nfa1UTR | gDNA |
nfa1 R3 | 5′-AACTCTTCACGAGCAAATGCCAAACGCTTTAAAGCAC-3′ | 361-397 in nfa1UTR | nfa1UTR | gDNA |
VS1 | 5′-GTTTGGACAAACCACAACTAGAATGCAGTG-3′ | 1599-1628 in pEGFP-C2 | nfa1 | gDNA |