TABLE 3.
Measurement of in vitro cytotoxicities of N. fowleri, N. gruberi, and transgenic N. gruberi for CHO cells by LDH release assay
| Group | Cytotoxicity (%) |
|---|---|
| CHOa | |
| CHO + N. fowleri trophozoitesb | 82.0 ± 2.1 |
| CHO + N. fowleri trophozoites + anti-Nfa1 Abc | 38.1 ± 4.7 |
| CHO + N. gruberi trophozoitesb | 17.7 ± 1.6 |
| CHO + N. gruberi trophozoites + anti-Nfa1 Ab | 17.3 ± 1.5 |
| CHO + N. gruberi trophozoites + G418d | 18.2 ± 0.4 |
| CHO + transgenic N. gruberi trophozoitesb | 55.8 ± 0.7 |
| CHO + transgenic N. gruberi trophozoites + anti-Nfa1 Ab | 39.8 ± 1.6 |
a
3 × 104 cells.
b
3 × 104 trophozoites.
c
Ab, antibody. 1:100 dilution.
d
1 mg/ml of G418 antibiotic.