FIG. 3.

Immunoblot of the total proteins of L. pneumophila with mouse monospecific anti-LaiA convalescent-phase sera. After suspension in SDS-PAGE sample buffer, samples were electrophoresed on an 8% running gel, blotted, and reacted as described in Materials and Methods. An open arrowhead indicates the full-length LaiA protein. Bands shown by closed arrowheads seem to be the degraded fractions of LaiA. Positions of molecular mass markers are indicated on the right. Lane 1, total cellular protein of 80-045; lane 2, total cellular protein of PK-treated 80-045; lane 3, total cellular protein of LAM0101.