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Confocal microscopy analysis of intracellular trafficking of Salmonella-containing vacuoles in macrophages and B lymphocytes. Monolayers of preactivated IC21 or bone marrow-derived macrophages from BALB/c mice or A-20 cells were infected with Salmonella enterica serovar Typhimurium (MOI, 1:10) for a period of 15 min. Selective images of colocalization with Lamp1 markers and bacteria within macrophages and B lymphocytes at 30 min and 24 h postinfection are shown.
