FIG. 4.

Salmonella CFU recovered from intracellular vesicles from IFN-γ-activated macrophages and B lymphocytes. Aliquots of the Percoll gradient fractions were cultured in LB agar plates to detect bacterial survival. On the left y axis, we present the percentage of total β-hexosaminidase activity, CFU numbers are displayed on the right y axis, and the x axis corresponds to the number of density gradient fractions. Panels A and C show gradients obtained from B lymphocytes. Panels B and D show data obtained from macrophages. One representative experiment out of three is presented.