FIG. 5.

S. enterica serovar Typhimurium organisms survive in B lymphocytes during primary in vivo infection. BALB/c mice were infected orogastrically (OG) with 1,000 CFU or intraperitoneally (IP) with 50 CFU. On days (d) 3 and 5 postinfection, splenocytes were harvested and macrophages and B cells were sorted based on the expression of Mac-1 (macrophages) or B220 (B lymphocytes). In panel A, we exhibit a representative dot blot analysis of the sorted B lymphocytes using a CD19 marker. In panel B, we show Salmonela CFU obtained from splenic B cells and macrophages. The white bars correspond to CFU obtained from OG infected mice, and black bars correspond to IP infected mice. Data are presented as means and standard deviations of the results from three independent experiments.