TABLE 1.
Plasmids and bacterial strains used in this study
| Plasmid or strain | Phenotype and description | Source |
|---|---|---|
| Plasmids | ||
| pCR-2.1 TOPO | Apr, Kmr | Invitrogen |
| pFLL80 | pCR 2.1-TOPO PG0791:PG0792 | 50 |
| pFLL86 | pUC 19: PG0791:PG0792 | This study |
| pFLL87 | pUC19: PG0791:PG0792:: ermF-ermAM | This study |
| pVA2198 | Spr, ermF-ermAM | 15 |
| Bacterial strains | ||
| P. gingivalis | ||
| W83 | Wild type | 1 |
| FLL93 | vimE-defective | 50 |
| FLL95 | vimF-defective | This study |
| E. coli | ||
| DH5α | F−φ80dlacZΔ M15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk−, mk+) phoA supE44 λ−thi-1 gyrA96 relA1 | Invitrogen |
| Top10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔ M15 ΔlacX74 recA1 ara139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |