FIG. 2.

Purification of AIDA translocator fusion proteins DT1 and DT2. The proteins were purified by IMAC as described in Materials and Methods. The final elution was performed with 250 mM imidazole in TNO-0.5. (A) The fractions were treated with sample buffer, analyzed by gradient SDS-PAGE (5 to 11% polyacrylamide), and stained with Coomassie brilliant blue or, after Western blotting, detected with antibodies directed against the AIDA translocator (anti-fp12). (B) Treatment with trypsin of the refolded DT1 and DT2 proteins generates the protease-resistant core region of the AIDA translocator (22, 24).