TABLE 1.
Plasmids encoding different AIDA (fusion) proteins
| Plasmid | Size (kb) | Antibiotic resistance | Descriptiona | Reference(s) |
|---|---|---|---|---|
| pCB3 | 5.6 | Ampr | Derivative of pGEM-4Z (Promega), encodes OspG fused to the AIDA translocator | Buddenborg, unpublished data |
| pDM1 | 9.7 | Kanr | Derivative of pGP1-2, encodes AAH and unprocessed AIDA | This study |
| pDT1 | 5.5 | Ampr | Derivative of pBR322, encodes signal peptide, N-terminal His6, and the AIDA translocator | 39 |
| pDT2 | 3.9 | Ampr | Derivative of pBR322, encodes N-terminal His6 and the AIDA translocator | 39 |
| pIB264 | 10.0 | Ampr | Derivative of pBR322, encodes AAH and native AIDA | 1 |
| pIB59 | 9.7 | Kanr | Derivative of pGP1-2, encodes AAH and native AIDA | Benz, unpublished data |
| pMK25 | 5.4 | Ampr | Derivative of pBR322, encodes 9-aa HA epitope fused to the AIDA translocator | 22, 24 |
a
AAH, autotransporter adhesin heptosyltransferase.