TABLE 2.
Antibodies used in this study
| Antibody | Dilution | Description | Reference or source |
|---|---|---|---|
| Anti-AIDA | 1:20,000/1:500a | Polyclonal rabbit serum directed against AIDA-I | 4 |
| Anti-fp12 | 1:20,000b | Polyclonal rabbit serum directed against the AIDA translocator (amino acids 847-1286) | 36 |
| Anti-MRGS-His6 | 1:5,000b | Monoclonal antibody (mouse) directed against the MRGS-His6 epitope | QIAGEN, Hilden, Germany |
| Anti-OmpA | 1:5,000b | Polyclonal rabbit serum directed against OmpA | U. Henning (MPI, Tübingen, Germany) |
| Anti-OmpF/C | 1:2,000b | Polyclonal rabbit serum directed against OmpF and OmpC | U. Henning (MPI, Tübingen, Germany) |
| Anti-Omp85 | 1:5,000b | Polyclonal rabbit serum directed against E. coli Omp85 | J. P. M. Tommassen (University of Utrecht) |
| Anti-HA | 1:2c | Monoclonal antibody directed against the 9-aa HA epitope of influenza virus hemagglutinin | Roche Diagnostics, Mannheim, Germany |
| Anti-OspG | 1:20,000/1:100a | Polyclonal rabbit serum directed against B. burgdorferi protein OspG | R. Wallich (DKFZ, Heidelberg, Germany) |
| Goat anti-mouse AP | 1:5,000b | Secondary antibody | QIAGEN, Hilden, Germany |
| Goat anti-mouse Au 12 nm | 1:25c | Anti-mouse antibody coupled to 12-nm Au particles | British Biocell International, Cardiff, Wales |
| Goat anti-rabbit Au 12 nm | 1:25c | Anti-rabbit antibody coupled to 12-nm Au particles | British Biocell International, Cardiff, Wales |
| Goat anti-mouse Cy3 | 1:100d | Secondary antibody | Jackson ImmunoResearche |
| Goat anti-rabbit APf | 1:5,000b | Secondary antibody | Jackson ImmunoResearche |
| Goat anti-rabbit Cy2 | 1:100d | Secondary antibody | Jackson ImmunoResearche |
| Goat anti-rabbit POg | 1:7,500b | Secondary antibody | Jackson ImmunoResearche |
a
The first dilution is the dilution used for western blotting, and the second dilution is the dilution used for immunofluorescence analysis.
b
Dilution used for western blotting.
c
Dilution used for immunogold labeling.
d
Dilution used for immunofluorescence analysis.
e
Jackson ImmunoResearch Laboratories, USA (via Dianova, Hamburg, Germany).
f
AP, alkaline phosphatase.
g
PO, peroxidase.