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. 2005 Jul;73(7):3983–3989. doi: 10.1128/IAI.73.7.3983-3989.2005

FIG. 4.

FIG. 4.

P. gingivalis strain cSMF1, complemented in trans with mfa1, expresses Mfa1 protein which is located on the cell surface and confers a coadhesion phenotype on the organism. (A) Immunoblot of whole-cell lysates. Lanes 1, strain 33277; lanes 2, SMF1 (Mfa1−); lanes 3, cSMF1 probed with antiserum to rMfa or to 33277 whole cells (1:10,000). Size standards (kDa) are shown in lane M. (B) ELISA of fixed cells of P. gingivalis strains 33277, SMF1, or cSMF1 probed with preimmune serum, antiserum to rMfa, or antiserum to 33277 whole cells. (C) Coadhesion of tritiated cells of P. gingivalis stains 33277, SMF1, or cSMF1 with S. gordonii cells adsorbed to a nitrocellulose membrane. Plot is the result of a curve-fitting algorithm using SigmaPlot V8. Error bars represent standard deviation (n = 3).